Product Type
ELISA kit
Measurement
Qualitative
Description
This is a qualitative enzyme immunoassay for the detection of antibodies to Trichinella, in samples of human serum or plasma. This test is intended to be performed by trained medical technologists only.
Sample Type
Human serum or plasma
Size
96 T
Kit Components
Test strips (MT PLATE): Microwells containing Trichinella ES antigens - 96 test wells in a test strip holder.
Enzyme conjugate (CONJ): 1 bottle containing 11 mL of protein A conjugated to peroxidase.
Positive control (CONTROL+): 1 vial containing 2 mL of a surrogate positive control.
Negative control (CONTROL-): 1 vial containing 2 mL of diluted human sera.
Chromogen (SUBS, TMB): 1 bottle containing 11 mL of the chromogen tetramethylbenzidine (TMB).
Wash concentrate (20x) (WASH BUF): 2 bottles containing 25 mL of concentrated buffer and surfactant.
Dilution buffer (SPECM DIL): 2 bottles containing 30 mL of buffered protein solution.
Stop solution (SOLN): 1 bottle containing 11 mL of 1 M phosphoric acid.
Enzyme conjugate (CONJ): 1 bottle containing 11 mL of protein A conjugated to peroxidase.
Positive control (CONTROL+): 1 vial containing 2 mL of a surrogate positive control.
Negative control (CONTROL-): 1 vial containing 2 mL of diluted human sera.
Chromogen (SUBS, TMB): 1 bottle containing 11 mL of the chromogen tetramethylbenzidine (TMB).
Wash concentrate (20x) (WASH BUF): 2 bottles containing 25 mL of concentrated buffer and surfactant.
Dilution buffer (SPECM DIL): 2 bottles containing 30 mL of buffered protein solution.
Stop solution (SOLN): 1 bottle containing 11 mL of 1 M phosphoric acid.
Materials Provided
Trichinella IgG ELISA Kit
Materials Required But Not Included In Kit
Micropipette
Squeeze bottle for washing strips (narrow tip is recommended)
Reagent grade (DI) water
Graduated cylinder
Sample dilution tubes
Absorbent paper
Squeeze bottle for washing strips (narrow tip is recommended)
Reagent grade (DI) water
Graduated cylinder
Sample dilution tubes
Absorbent paper
Suggested Materials
ELISA plate reader with a 450 nm and a 620 - 650 nm filter (optional if results are read visually).
Storage
Reagents, strips and bottled components should be stored at 2-8 °C.
Squeeze bottle containing diluted wash buffer may be stored at room temperature (15-25 °C).
Squeeze bottle containing diluted wash buffer may be stored at room temperature (15-25 °C).
Regulatory Status
For research use only.