Fleas are small, wingless, parasitic insects that are good jumpers. Adults usually live on mammals and a few on birds. Fleas are not only a health hazard to animals, but also a serious threat to the quality of the living environment and the health of people. Adult fleas of both sexes have a blood-sucking habit, and bites often cause local tissue metamorphosis, producing papules of different sizes and itching in severe cases, and can also cause iron deficiency anemia. Fleas are important vectors for the transmission of many important infectious diseases, including tapeworm disease, plague, renal syndrome hemorrhagic fever, endemic typhus, and Baltons disease.

Transcriptome sequencing can comprehensively resolve the mRNA and non-coding RNA information of fleas. Ace Therapeutics provides transcriptome sequencing and analysis services for flea. It simultaneously obtains transcript structure and abundance information, probes structural variation of flea pathogenesis-related genes, mines differentially expressed genes to analyze pathogenesis, and reveals the molecular mechanism of specific biological processes at the overall level.

Transcriptome Sequencing Service Options for Fleas

We offer transcriptome sequencing services for flea, including but not limited to the following, which you can choose flexibly according to your needs.

• Absolute quantitative transcriptome sequencing service for flea
• Relative quantitative transcriptome sequencing services for flea

We provide absolute quantitative transcriptome sequencing services for flea, using mainstream UMI marker technology to eliminate the interference of PCR amplification preferences on quantification and to truly reflect the expression abundance of transcripts in flea samples.

We also provide relatively quantitative transcriptome sequencing services (next-generation and third-generation transcriptome) for fleas to quantify changes in expression levels of each transcript during their growth and under different conditions, to achieve gene annotation and screening of fleas and to improve their genome annotation; to achieve differential gene expression and changes in alternative splicing events. To provide theoretical basis for revealing its pathogenic mechanism and finding drug targets.

• Whole transcriptome sequencing service for flea

We also provide whole transcriptome sequencing service for fleas, which can simultaneously analyze mRNA, lncRNA, circRNA and microRNA individually for the same sample, as well as combine multiple RNA analysis, endogenous competitive RNA integration analysis, and molecular marker screening. We also provide independent sequencing services for mRNA, lncRNA, circRNA, and small RNA to explore the potential regulatory network mechanism and reveal the transcriptional regulation problem comprehensively. These technologies are mainly applied to the study of immune mechanism, developmental evolution, pathogenesis and drug targets of fleas.

• Dual RNA sequencing service for flea and host

There are complex interactions between fleas and host cells. Dual RNA-seq can simultaneously analyze the dynamic changes of gene expression between two (or more) mutualistic species, and obtain the regulatory relationships of genes between species through mutualistic model maps to obtain the mechanisms of inter-species interactions. We provide dual RNA-seq service to directly study the dynamic interactions between fleas and hosts at the molecular level, which helps to better understand the physiological changes during infection and thus reveal potential new targets or biomarkers. We can process the interaction information quickly and flexibly in the massive bioinformatics data.

Service Details

Sample requirements: each service is with independent sample requirements, please consult our technical engineers or sales staff for details.
Delivery content: experiment report.
Test fee: please get it through online inquiry.

Service Flow

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service