Ascariasis is one of the most common parasitic diseases, which causes serious harm to human. Swine ascariasis is a common and serious parasitic disease of pigs in production, which can cause digestive problems in piglets, reduce the feed rate and increase the cost of breeding; in serious cases, a large number of worms block the intestinal canal and lead to death, bringing huge losses to farmers. Human ascariasis is also the most widespread human helminthic disease in the world, with WHO estimating that there are 1.3 billion patients worldwide, especially in developing countries such as Asia, Africa and Latin America. Parasitism of Ascaris suum can cause damage to the intestinal mucosa, leading to digestive and absorption disorders, or other complications that can be severe and even fatal, posing a great threat to human health. Therefore, it would be of great importance to study the prevention and control of such diseases.

By sequencing, assembling, annotating and analyzing the transcriptome level of ascarid, we tried to understand its genetic characteristics and some pathogenic mechanisms from the transcriptome level, which is very important for the prevention and treatment of ascaris. By further mining the transcriptome information, we hope to provide more valuable information for the diagnosis, prevention and treatment of porcine ascariasis and human ascariasis.

Ace Therapeutics provides transcriptome level sequencing and analysis services for ascarids, aiming to classify the complete transcripts, including mRNAs, non-coding RNAs and small RNAs in ascarid cells under specific developmental stages or physiological conditions. Understanding the ascarid transcriptome is essential for interpreting the functional components of its genome, revealing the molecular composition of cells and tissues, and understanding development and disease. It has been widely used in basic research and drug development in ascarid.

Transcriptome Sequencing Service Options for Ascaris

• Transcriptome sequencing service for ascarid without reference genome

We use this technical service to obtain raw sequencing data of RNA transcribed from an ascarid in a specific tissue or cell in a certain state, and assemble it after quality control to obtain unigene; and use it as the reference sequence of this ascarid for subsequent functional annotation, SNP and SSR marker development, as well as expression analysis and functional enrichment analysis of differential genes in multiple samples; in order to discover new functional genes and provide technical support for further research.

• Transcriptome sequencing service for ascarid with reference genome

We use this technology service to quickly obtain the sequence of transcripts transcribed from a specific tissue or cell of ascid in a certain state and compare it with the reference genome to analyze gene expression levels, differentially expressed genes, alternative splicing, gene fusion, gene structure optimization, SNP, SSR and prediction of new transcripts. In-depth study of the gene functions of ascarid will guide further research of the network regulatory mechanism.

• Full-length transcriptome sequencing service for ascarid

We also provide full-length transcriptome sequencing and analysis services for ascarid. We can take advantage of the long read length sequencing platform to obtain more accurate gene sets, discover new genes and transcript isoforms, and accurately identify variable splicing and gene fusions, as well as assist in de novo gene annotation of the ascarid genome to obtain better gene annotation results. Our platform is well run and our experimenters and information analysts are experienced.

• mRNA sequencing service for ascarid
• LncRNA sequencing service for ascarid
• CircRNA sequencing service for ascarid
• Small RNA sequencing service for ascarid

We also provide sequencing and analysis services for lncRNA-Seq, small RNA-Seq, circRNA-Seq and other types of transcriptomics, enabling the identification, quantification and functional analysis of lncRNA, small RNA and circRNA. We can also perform multi-omics integration analysis, such as lncRNA+ small RNA+ circRNA, lncRNA+ small RNA, lncRNA-Seq+ Small RNA+ circRNA. We can extend the study of gene regulation mechanism to a three-dimensional model of "net", which helps to gain a relatively comprehensive understanding of the regulatory mechanisms of ascarid growth and disease development, the search for biomarkers, the study of targeted drugs, and many other biological problems.

• Whole transcriptome sequencing service for ascarid

With more than 10 years of project experience, we have the ability to handle complex samples and rich experience in library construction to ensure the quality of library construction. We also have featured advanced analysis, which can experiment lncRNA regulatory mechanism prediction, co-expression analysis of lncRNA and mRNA, combined analysis of lncRNA, mRNA and circRNA, etc. We also have the flexibility to perform customized information analysis in conjunction with customer needs.

Service Details

Sample requirements: each service is with independent sample requirements, please consult our technical engineers or sales staff for details.
Delivery content: experiment report.
Test fee: please get it through online inquiry.

Service Flow

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service