Product Type
ELISA kit
Measurement
Semi-quantitative
Description
This is a semi-quantitative enzyme immunoassay for the detection of IgM antibodies to Toxocara, in samples of human serum or plasma. This test is intended to be performed by trained medical technologists only.
Sample Type
Human serum or plasma
Size
96 T
Kit Components
Test strips (MT PLATE): Microwells containing Toxocara excretory antigens - 96 test wells in a test strip holder.
Enzyme conjugate (CONJ): 1 bottle containing 11 mL of anti-human IgM (μ chain specific) conjugated to peroxidase.
Positive control (CONTROL+): 1 vial containing 2 mL of diluted surrogate positive control.
Negative control (CONTROL-): 1 vial containing 2 mL of diluted human sera.
Chromogen (SUBS, TMB): 1 bottle containing 11 mL of the chromogen tetramethylbenzidine (TMB).
Wash concentrate (20x) (WASH BUF): 2 bottles containing 25 mL of concentrated buffer and surfactant.
Dilution buffer (SPECM DIL): 2 bottles containing 30 mL of buffered protein solution with RF Absorbent.
Stop solution (SOLN): 1 bottle containing 11 mL of 1 M phosphoric acid.
Enzyme conjugate (CONJ): 1 bottle containing 11 mL of anti-human IgM (μ chain specific) conjugated to peroxidase.
Positive control (CONTROL+): 1 vial containing 2 mL of diluted surrogate positive control.
Negative control (CONTROL-): 1 vial containing 2 mL of diluted human sera.
Chromogen (SUBS, TMB): 1 bottle containing 11 mL of the chromogen tetramethylbenzidine (TMB).
Wash concentrate (20x) (WASH BUF): 2 bottles containing 25 mL of concentrated buffer and surfactant.
Dilution buffer (SPECM DIL): 2 bottles containing 30 mL of buffered protein solution with RF Absorbent.
Stop solution (SOLN): 1 bottle containing 11 mL of 1 M phosphoric acid.
Materials Provided
Toxocara IgM ELISA Kit
Materials Required But Not Included In Kit
Micropipette
Reagent grade (DI) water
Graduated cylinder
Timer
Tubes for serum dilution
Reagent grade (DI) water
Graduated cylinder
Timer
Tubes for serum dilution
Suggested Materials
ELISA plate reader with a 450 nm and a 620 - 650 nm filter.
Storage
Reagents, strips and bottled components should be stored at 2-8 °C.
Squeeze bottle containing diluted wash buffer may be stored at room temperature (15-25 °C).
Squeeze bottle containing diluted wash buffer may be stored at room temperature (15-25 °C).
Regulatory Status
For research use only.
