Plasmodium is a sporozoite transmitted by Anopheles mosquitoes and is the causative agent of malaria. There are many species of Plasmodium, but there are four main species of plasmodium parasitizing humans, namely Plasmodium vivax Grassi & Feletti, Plasmodium malariae Laveran, Plasmodium falciparum Welch and Plasmodium ovale Stephens. The life histories of the four species of plasmodium parasitizing humans are basically the same, requiring two hosts, human and Anopheles. In humans, they are parasitized in liver cells and erythrocytes and undergo schizogony (proliferation). Development in the human body is divided into extra-erythrocytic stage (intrahepatocyte development) and intra-erythrocytic stage (intra-erythrocytic development and proliferation and male and female gametophyte formation).

Ace Therapeutics specializes in parasitology and has established a parasite culture technology platform that provides culture services for a wide range of parasites. We provide in vitro culture services for plasmodium, aiming to facilitate its experimental diagnosis, parasite morphology, life history (ecology), pathogenesis, epidemiological patterns, immunogenetic characteristics, preservation of insect species, and development of vaccines.

Service Options for Plasmodium Culture

Plasmodium culture can be divided into intra-erythrocytic and extra-erythrocytic stages. Of the four human Plasmodium species in the intra-erythrocytic phase culture only Plasmodium falciparum can be successfully cultured in vitro for long periods of time. We offer both intra-erythrocytic and extra-erythrocytic plasmodium cultures, so you have the flexibility to choose according to your needs.

• Intra-erythrocytic plasmodium culture
• Extra-erythrocytic plasmodium culture

Service Details

Culture medium: basic culture medium such as RPMI1640.
Sample type: "O" human erythrocytes, human serum.
Other substances: HEPES, glutathione, gas mixtures (3% O₂, 4% CO₂ and 93% N₂) and sterile cell culture bottles.
Quality testing: we will also aspirate a small number of precipitated cells at regular intervals for staining with Kirschner's stain to see how the worms are growing.
Delivery content: plasmodium with culture medium and report.
Test fee: please get it through online inquiry

Service Flow

1. Communicate your project requirements to us via online inquiry or telephone to obtain the experimental protocol.
2. Sign the contract and pay the deposit.
3. Fill out the sample order and send the sample.
4. Pre-experimentation. Optimize conditions based on pre-experiments, customer needs, culture experience and standard methods.
5. Plasmodium culture and preparation of project reports.
6. Delivery of cultured plasmodium and report.

Our Advantages

• We offer 2 periods of plasmodium culture services, intra-erythrocytic and extra-erythrocytic, for you to choose flexibly.
• Professional technicians with extensive culture experience and proficiency in plasmodium in vitro culture.
• In addition to providing plasmodium in vitro culture services, we also provide plasmodium testing (plasmodium blood smear microscopy, ELISA, IFA, PCR, fluorescence RT-PCR, etc.) services and animal model development services.
• High quality service at affordable prices.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service