Endophytic bacteria have been found in all plants studied so far, and they can be found in various parts of plants, such as roots, stems, leaves, flowers, and fruits. It has been found that endophytic bacteria can infest the host plant through various pathways, in addition to spreading with plant genetics. Endophytic bacteria, mainly including fungi, bacteria, and actinomycetes, live in a special environment within the host for a long time and evolve synergistically with the host, forming a symbiotic relationship during the evolutionary process. On the one hand, endophytic bacteria can absorb nutrients from the host for their own growth needs, and on the other hand, endophytic bacteria play an important role in the growth and development of the host and in the evolution of the system. There are complex interactions between endophytic bacteria and host plants, including antagonism, neutralism, commensalism, and mutualism.

Ace Therapeutics provides a comprehensive service for the study of plant endophytes and host fungi interactions to help you gain a deeper understanding of plant-parasitic nematode-host interactions.

Service Options for Plant Endophyte-Host Fungal Interaction Study

1. ELISA and NIRS

We offer enzyme-linked Immunosorbent assay (ELISA) and near-infrared spectroscopy (NIRS) services for the quantitative detection of endophytic fungi.

2. Gene microarray and protein microarray

We provide gene microarray technology services and protein microarray technology services, which are mainly used to study the mechanism of action of endophytes on host plants. We provide gene microarrays for both gene expression profiling and functional gene screening of Phytophthora endophytes, and also for gene expression profiling of Phytophthora-infected hosts, providing a faster and better way to investigate the different mechanisms of host immune response processes caused by Phytophthora endophytes. Protein microarrays can play an important role in parasitic disease detection as well as in anti-parasitic vaccine target screening and drug development. Their widespread use will greatly accelerate the pace of research in the field of parasitology. Please consult us for details on chip substrate specifications, the number of dots on the chip, dot diameter, technical repetition, and other specific information.

3. Bimolecular fluorescence complementation (BiFC)

We provide bi-molecular fluorescence complementation technology services for the study of plant endophyte-host interactions. Our service process includes the following.

1) Construction of plasmids containing the target proteins (client needs to confirm whether the target protein is attached to the N-terminal or C-terminal end of the fluorescent protein.)
2) Transformation of Agrobacterium by recombinant target plasmid.
3) Agrobacterium bacteriophage injection into tobacco leaves.
4) Confocal microscope observation and photography.

Upon completion of the project, we will deliver a full lab report with detailed steps and results, along with the original images (including YFP, DIC, Merge), as well as the constructed plasmids and Agrobacterium.

Note: In addition, there are other technical services available for you to choose from, please consult us for details.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service