Product Type
Kit
Unit Size
150 T
Description
Employing Taqman probe-based real-time PCR, the Naegleria qPCR kit detects Naegleria genomes in samples such as brain tissue and cerebrospinal fluids.
Components
Naegleria primers and probe mix (Naegleria PPM)
Positive control
Internal extraction control primers and probe mix (IEC PPM)
Internal extraction control (IEC DNA)
Nuclease free water
Template preparation buffer
Positive control
Internal extraction control primers and probe mix (IEC PPM)
Internal extraction control (IEC DNA)
Nuclease free water
Template preparation buffer
Technology
Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit
DNA
Sample Material
Clinical samples (e.g. brain tissue, cerebrospinal fluids)
Gene Target
18S ribosomal RNA (18S) gene
Detection Limit
10 to <100 copies per reaction
Sensitivity & Specificity
Ct value 28±3 with probability of 95%
Controls Included
Internal extraction control (IEC) as inhibition control.
Positive control.
Positive control.
Channels
FAM channels detect pathogen amplicons.
VIC/HEX channel detects IEC amplicons.
VIC/HEX channel detects IEC amplicons.
Storage Conditions
Shipped in room temperature, store at -20°C on arrival.
Regulatory Status
For research use only.
