Mycoparasitism is a form of parasitism between mycoparasitic fungi and host fungi and is a fungal-fungal interaction that is prevalent in nature. It has been shown that the interaction between mycoparasitic mycorrhizal fungi and host fungi is divided into four sequential processes: target location, recognition, contact and penetration, and nutrient acquisition, in that order. There are large differences in this process between dead and living nutrient-parasitic mycorrhizal organisms. In recent years, there has been great progress in the basic biochemical study of the interaction between dead nutrient bacteria parasitic mycorrhizal organisms and their host organisms, mainly focusing on the production of wall lysozyme by the bacteria parasitic mycorrhizal organisms and its induction and mechanism of action. The mechanism of mutual recognition between contact-viable nutrient-rich bacterial parasitic organisms and host organisms is still lacking in research. In view of the research on the interaction between mycoparasitic fungi and host fungi, we should use more cutting-edge science and technology to explore the mechanism of action.

Ace Therapeutics provides a comprehensive service for the study of mycoparasites and host fungi interactions to help you gain a deeper understanding of plant-parasitic nematode-host interactions.

Service Options for Mycoparasite and Host Fungal Interaction Study

1. Interaction proteome

The proteins between mycoparasites and host fungi form complexes to perform their functions. The Interactome (Interactome) allows for the simultaneous identification of target proteins and their interacting proteins to construct protein interaction profiles. The technique is applicable to both mass spectrometry analysis of protein mixtures separated by SDS-PAGE followed by in-gel enzymatic digestion and mass spectrometry analysis of liquid enzymatic digestion of protein mixtures in solution. Applicable samples include protein mixtures in samples obtained by LC-MS/MS techniques for IP, Co-IP, Pull-down, and other techniques. We provide this service with high accuracy, high specificity, and short project cycle time.

2. Yeast one hybrid and yeast two-hybrid

Yeast one hybridization (Y1H) can be used to study nucleic acid-protein interactions between mycoparasites and host fungi, which is important for resolving gene expression regulation, identifying DNA binding sites to discover potential binding protein genes, and analyzing DNA binding structural domain information.

The yeast two-hybrid system (Y2H) is a good research method for the identification and detection of protein interactions between mycoparasites and host fungi because of its high sensitivity, power, and wide applicability.

3. Immunoprecipitation

We provide Co-Immunoprecipitation (CO-IP) services to detect whether two target proteins interact in vivo or to identify the interacting protein of a protein in vivo, by precipitating an immune complex with the protein that interacts with the antibody target protein in the sample of the mycoparasites and host fungi.

4. GST pull-down

We offer GST pull-down assays for the study of mycoparasites and host fungi, using specific secondary antibodies to avoid signal interference from antibody heavy chains and to significantly improve the detection results. Both "decoy proteins" and "capture proteins" can be obtained from cell lysates, purified proteins, expression systems, and in vitro transcription-translation systems. This method is simple and easy to use.

Sample Requirements and Delivery Content

• Interaction proteome

The client provides the samples to be identified.

We provide a full lab report with specific experimental procedures and proteome identification, quantitative results, and bioinformatics analysis (including differential protein expression analysis, identification of interacting proteins, prediction of protein network interactions based on STRING database, protein GO functional classification, COG functional annotation, pathway annotation).

• Y1H and Y2H

Tissue/cell provided by the customer, if library screening is to be performed, then promoter sequence/protein sequence/gene sequence/plasmid is also required.

We deliver yeast library broth, images of library PCR identification results, all original images from the screening process, and the sequencing results of all interacting prey proteins obtained from the screen.

• GST pull-down

Constructs expressing purified target proteins with associated decoy proteins (also optionally constructed by us), or cell lysate supernatants containing decoy proteins or other; primary antibodies for detection (also optionally provided by us). Samples should be frozen at -20°C and shipped in ice packs.

Full lab report, SDS-PAGE, and western blot results are provided.

• CoIP

The customer provides the sample to be tested, a primary antibody (also optionally provided by us). Animal tissue ≥ 400 mg, plant tissue ≥ 2 g, cells ≥ 2x10⁷.

WB films/photographs and experimental reports are provided.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service