ELISA kit

This is an enzyme immunoassay for the detection of antibodies to Leptospira biflexa (serovar patoc 1) for the serological confirmation of infections in serum and plasma. This test is intended to be performed by trained laboratory personnel only.

Serum and plasma

96 T

Test strips (MT PLATE): Microwells containing Leptospira antigen - 96 test wells in a test strip holder.
Enzyme conjugate (CONJ): 1 bottle containing 11 mL of anti-human IgG antibody conjugated to peroxidase.
Positive control (CONTROL+): 1 vial containing 2 mL of diluted surrogate positive control.
Negative control (CONTROL-): 1 vial containing 2 mL of diluted negative human serum.
Chromogen (SUBS, TMB): 1 bottle containing 11 mL of the chromogen tetramethylbenzidine (TMB).
Wash concentrate (20x) (WASH BUF): 2 bottles containing 25 mL of concentrated buffer and surfactant.
Dilution buffer (SPECM DIL): 2 bottles containing 30 mL of buffered protein solution.
Stop solution (SOLN): 1 bottle containing 11 mL of 1 M phosphoric acid.

IVD Leptospira IgG Microwell ELISA Kit

Micropipette
Squeeze bottle for washing strips (narrow tip is recommended)
Reagent grade (DI) water
Graduated cylinder
Sample dilution tubes
Absorbent paper

ELISA plate reader with a 450 nm and a 620-650 nm filter (optional if results are read visually).

Reagents, strips and bottled components should be stored at 2-8 °C.
Squeeze bottle containing diluted wash buffer may be stored at room temperature (15-25 °C).

For research use only.