Product Type
ELISA kit
Description
For the screening of serum antibodies, primarily IgM, for visceral Leishmania using the ELISA technique.
Sample Type
Serum
Size
96 T
Kit Components
Test strips (MT PLATE): Microwells containing Leishmania antigens - 96 test wells in a test strip holder.
Enzyme conjugate (CONJ): 1 bottle containing 11 mL of anti-human IgM (µ chain) Peroxidase (HRP) in a stabilizing buffer with thimerosal.
Positive control (CONTROL+): 1 vial containing 2 mL of diluted surrogate positive in buffer with thimerosal.
Negative control (CONTROL-): 1 vial containing 2 mL of diluted Leishmania-negative human sera in buffer with thimerosal.
Chromogen (SUBS, TMB): 1 bottle containing 11 mL of the chromogen tetramethylbenzidine (TMB).
Wash concentrate (20x) (WASH BUF): 2 bottles containing 25 mL of concentrated buffer and surfactant.
Dilution buffer (SPECM DIL): 2 bottles containing 30 mL of buffered protein solution.
Stop solution (SOLN): 1 bottle containing 11 mL of 1 M phosphoric acid.
Enzyme conjugate (CONJ): 1 bottle containing 11 mL of anti-human IgM (µ chain) Peroxidase (HRP) in a stabilizing buffer with thimerosal.
Positive control (CONTROL+): 1 vial containing 2 mL of diluted surrogate positive in buffer with thimerosal.
Negative control (CONTROL-): 1 vial containing 2 mL of diluted Leishmania-negative human sera in buffer with thimerosal.
Chromogen (SUBS, TMB): 1 bottle containing 11 mL of the chromogen tetramethylbenzidine (TMB).
Wash concentrate (20x) (WASH BUF): 2 bottles containing 25 mL of concentrated buffer and surfactant.
Dilution buffer (SPECM DIL): 2 bottles containing 30 mL of buffered protein solution.
Stop solution (SOLN): 1 bottle containing 11 mL of 1 M phosphoric acid.
Materials Provided
Visceral Leishmania IgM Serology Microwell ELISA Kit
Materials Required But Not Included In Kit
Pipettes
Squeeze bottle for washing strips
DI water
ELISA plate reader with a 450/620-650 nm filter (optionally, results can be read visually).
Tubes for serum dilutions
Squeeze bottle for washing strips
DI water
ELISA plate reader with a 450/620-650 nm filter (optionally, results can be read visually).
Tubes for serum dilutions
Storage
Reagents, strips and bottled components should be stored at 2-8 °C.
Squeeze bottle containing diluted wash buffer may be stored at room temperature (15-25 °C).
Squeeze bottle containing diluted wash buffer may be stored at room temperature (15-25 °C).
Regulatory Status
For research use only.
