Leishmania protozoa refers to the trypanosomes of the genus Leishmania, which have a protoflagellate and amastigote life history. The former is parasitic in the digestive tract of arthropods (whiteflies) and the latter is parasitic in mononuclear macrophages of vertebrates and is transmitted by whiteflies. Leishmania protozoa infects humans, dogs, cats, cattle, horses, and sheep. Test animals such as rats, dolphins and monkeys are also susceptible.

Leishmaniasis is a human-animal parasitic disease caused by various protozoa of the genus Leishmania and is transmitted between arthropods and mammals. The clinical pathology of the disease is characterized by irregular high fever, emaciation, anemia, leukopenia, enlargement of the liver, spleen, and lymph nodes, and the formation of hypertrophic or ulcerative lesions in the skin and mucous membranes.

Ace Therapeutics specializes in parasitology and has established a parasite culture technology platform that provides culture services for a wide range of parasites. We provide in vitro culture services for leishmania, aiming to facilitate its experimental diagnosis, morphological studies, life history (ecology), pathogenesis, epidemiological patterns, immunogenetic characteristics, and preservation of the species.

Service Options for Leishmaniasis Culture

We offer 2 periods of leishmania protozoa culture service, namely protoflagellate culture and amastigote culture, which you can choose flexibly according to your needs.

• Protoflagellate culture service

Type of medium: NNN(3N) medium and USMARU medium.
Sample type: biopsy specimens of skin or tissue, bone marrow.
There are many strains of leishmania protozoa that can also be successfully cultured in a mixture of some mammalian cell cultures, such as MEM, RPMI 1640 or 199 medium.

• Amastigote culture service

Cultured in macrophage culture strain J774G8
Cultured in macrophages isolated directly from peripheral blood

Service Details

Service items: protoflagellate culture service, amastigote culture service, protoflagellate culture & amastigote culture services
Delivery content: leishmania with culture medium
Test fee: please get it through online inquiry

Service Flow

1. Communicate your project requirements with us via online inquiry or by phone.
2. Sign the contract and pay the deposit.
3. Fill out the sample order and send the sample.
4. Initial test of samples. Optimize the conditions according to the customer's needs, sample characteristics, culture experience and standard methods.
5. Conduct experiments, obtain cultured parasites and make test report.
6. Delivery of the report and leishmania with culture medium.

Our Advantages

• 2 periods of leishmania protozoa culture service, with the flexibility to choose as needed.
• Professional technicians with extensive culture experience and proficiency in leishmania in vitro culture.
• In addition to providing leishmania in vitro culture services, we also provide leishmania testing services and animal model development services.
• Short project cycle and competitive pricing.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service