Product Type
ELISA kit
Measurement
Quantification, qualitative
Description
Toxoplasma gondii IgG ELISA kit is an enzyme-linked immunosorbent assay for the detection of IgG antibodies to Toxoplasma gondii.
Reactivity
Human
Sample Type
Human serum and plasma
Size
96 T
Detection Method
Colorimetric
Kit Components
Microtitre plate: Coated with antigen, 12 x 8 wells in bag with desiccant. 96 wells
Negative control (Calibrator 1): 0.1 IU/mL, solution containing no specific human antibodies, ready to use 2 mL
CUT-OFF (Calibrator 2): 6 IU/mL, solution containing specific human antibodies in cut-off concentration, ready to use. 3 mL
Positive control (Calibrator 3): 60 IU/mL, solution containing specific human antibodies, ready to use. 2 mL
Calibrator 4: 240 IU/mL, solution containing specific human antibodies, ready to use. 2 mL
Conjugate: Solution containing peroxidase labelled animal immunoglobulin to human IgG, ready to use. 15 mL
Sample diluent 5: Buffer with protein stabilizers, ready to use. 105 mL
TMB-complete 2: Chromogenic substrate solution containing TMB/H2O2, ready to use. 15 mL
Wash solution: 20x concentrated buffer. 75 mL
Stop solution: Acid solution, ready to use. 15 mL
Avidity solution 1: Stabilised urea solution. 7 mL
Negative control (Calibrator 1): 0.1 IU/mL, solution containing no specific human antibodies, ready to use 2 mL
CUT-OFF (Calibrator 2): 6 IU/mL, solution containing specific human antibodies in cut-off concentration, ready to use. 3 mL
Positive control (Calibrator 3): 60 IU/mL, solution containing specific human antibodies, ready to use. 2 mL
Calibrator 4: 240 IU/mL, solution containing specific human antibodies, ready to use. 2 mL
Conjugate: Solution containing peroxidase labelled animal immunoglobulin to human IgG, ready to use. 15 mL
Sample diluent 5: Buffer with protein stabilizers, ready to use. 105 mL
TMB-complete 2: Chromogenic substrate solution containing TMB/H2O2, ready to use. 15 mL
Wash solution: 20x concentrated buffer. 75 mL
Stop solution: Acid solution, ready to use. 15 mL
Avidity solution 1: Stabilised urea solution. 7 mL
Materials Required But Not Included In Kit
Single and multichannel pipettes
Disposable tips
Microplate washer
Timer
Incubator (37°C)
Microplate reader
Disposable tips
Microplate washer
Timer
Incubator (37°C)
Microplate reader
Sample Volume
100 μL
Label
HRP-conjugate
Calibration Range
0.1 - 240 IU/mL
Storage
Store the kit at 4°C. Do not freeze.
Regulatory Status
For research use only.
