Product Type
ELISA kit
Measurement
Qualitative
Description
Toxoplasma gondii IgE ELISA kit is an enzyme-linked immunosorbent assay for the detection of IgE antibodies to Toxoplasma gondii.
Reactivity
Human
Sample Type
Human serum and plasma
Size
96 T
Detection Method
Colorimetric
Kit Components
Microtitre plate: Coated with antigen, 12 x 8 wells in bag with desiccant. 96 wells
Negative control: Solution containing no specific human antibodies, ready to use. 2 mL
CUT-OFF: Solution containing specific human antibodies in cut-off concentration, ready to use. 3 mL
Positive control: Solution containing specific human antibodies, ready to use. 2 mL
Tracer: Lyophilisate containing T. gondii antigen and conjugate. 3 pcs
Conjugate: Solution containing peroxidase labelled animal immunoglobulin to human IgA, ready to use. 15 mL
Sample diluent 3: Buffer with protein stabilizers, ready to use. 105 mL
TMB-complete 2: Chromogenic substrate solution containing TMB/H2O2, ready to use. 15 mL
Tracer diluent: 13 mL
Wash solution: 20x concentrated buffer. 75 mL
Stop solution: Acid solution, ready to use. 15 mL
Negative control: Solution containing no specific human antibodies, ready to use. 2 mL
CUT-OFF: Solution containing specific human antibodies in cut-off concentration, ready to use. 3 mL
Positive control: Solution containing specific human antibodies, ready to use. 2 mL
Tracer: Lyophilisate containing T. gondii antigen and conjugate. 3 pcs
Conjugate: Solution containing peroxidase labelled animal immunoglobulin to human IgA, ready to use. 15 mL
Sample diluent 3: Buffer with protein stabilizers, ready to use. 105 mL
TMB-complete 2: Chromogenic substrate solution containing TMB/H2O2, ready to use. 15 mL
Tracer diluent: 13 mL
Wash solution: 20x concentrated buffer. 75 mL
Stop solution: Acid solution, ready to use. 15 mL
Materials Required But Not Included In Kit
Single and multichannel pipettes
Disposable tips
Microplate washer
Timer
Incubator (37°C)
Microplate reader
Disposable tips
Microplate washer
Timer
Incubator (37°C)
Microplate reader
Sample Volume
100 μL
Label
HRP-conjugate
Storage
Store the kit at 4°C. Do not freeze
Regulatory Status
For research use only.
