Intracellular parasites are capable of growing and reproducing within host cells, and there are two main types, parthenogenic and specialized. Parthenogenic intracellular parasites can survive and reproduce inside and outside the host cells. Specialized intracellular parasites require host cells to survive and reproduce. Many of these types of cells require specialized host types and host cell invasion occurs in different ways.

Bacteria that can be parthenogenic intracellular parasites include Bartonella henselae, Francisella tularensis, Listeria monocytogenes, Salmonella Typhi, Brucella, Legionella, Mycobacterium Nocardia, Neisseria, Rhodococcus equi, Yersinia, Staphylococcus aureus. Fungi that can be parthenogenic intracellular parasites include Histoplasma capsulatum, Cryptococcus neoformans, and Blastomyces dermatitidis.

Human specialized intracellular parasites include viruses, certain bacteria (Chlamydia), rickettsiae, beriberi, certain species of mycobacteria (Mycobacterium leprae), phagocytophagous anaerobes, certain protozoa (Apicomplexans, trypanosomes), certain fungi (Yersinia pneumocystis), etc.

Currently, the mechanisms of intracellular parasite-host interactions are not yet systematically studied but are mostly focused on the level of revealing phenomena. However, along with the continuous in-depth research on the mechanisms and pathways of cellular autophagy, the mechanisms of intracellular parasite infections and interactions with hosts will continue to be clarified. Ace Therapeutics provides one-stop integrated technical services for studying intracellular parasite-host interactions to meet our clients' related research needs.

Service Options for Intracellular Parasites-Host Interaction Research

1. RNA pull-down and DNA pull-down

Protein and RNA/DNA interactions are central to host cell and intracellular parasite functions, such as protein synthesis, mRNA assembly, viral replication, and cell developmental regulation. We provide technical services for RNA pull-down and DNA pull-down assays, with WB validation or mass spectrometry to identify protein types.

2. AP-SWATH

We offer an affinity purification (AP) approach to study the interaction between intracellular parasites and the cellular proteome, with specially designed purification tags that capture specific proteins and their molecular chaperones under conditions very close to physiological conditions.

3. scRNA-seq

We provide scRNA-seq technology services, which bring new methods and convenient means for cell type identification, the differential response of signaling pathways, molecular regulatory networks, and cell heterogeneity studies. It helps to understand the interactions behavior and mechanism of intracellular parasites and host cells.

We also provide technical services for dual RNA-seq, dual luciferase, ChIP, EMSA, Y1H, etc. These technical services can help to understand the interactions between intracellular parasites and host cells, such as whether they acquire humoral immune responses, whether drugs can cross the host cell membrane, with or without other nutrients, and whether they can recruit host functions for their own needs.

Sample Types

• For scRNA-seq and dual RNA-seq services, samples can be RNA of intracellular parasites and host cells.
• For RNA pull-down and DNA pull-down services, samples can be RNA, DNA, and protein, or intracellular parasites and host cells.
• For AP-SWATH, samples can be proteomes of intracellular parasites and host cells.

Service Details

• Service fee: please get it through online inquiry.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service