Product Type
Kit
Unit Size
100 T
Description
The Leishmania spp qPCR kit has been created specifically for the in vitro identification of Leishmania spp. genomes. It is engineered to offer the widest possible detection range while maintaining specificity for Leishmania spp. Therefore, this kit is tailored to accurately detect and differentiate this genus's presence in an in vitro setting. Through a thorough bioinformatic analysis using all reference data within the NCBI database, the primer and probe sequences exhibit significant homology (>95%) with a diverse array of Leishmania genomes from species such as Leishmania infantum, Leishmania donovani, Leishmania tropica, Leishmania killicki and Leishmania aethiopica, while not detecting other closely related genera.
Nucleic Acid Target
DNA
Compatibility
All standard real-time thermal cyclers
Target Species
Leishmania species
Detection Method
Probe based
Features
Free automatic data analyzer (Fastfinder)
Components
qPCR master mix (2x)
qPCR master mix reconstitution buffer
PPMix (10x)
NTC
Positive control
DNA internal extraction control (IEC)
qPCR master mix reconstitution buffer
PPMix (10x)
NTC
Positive control
DNA internal extraction control (IEC)
Format
Lyophilized
Technology
Predesigned qPCR assays
Sample Material
DNA
Protocol Time
Approx. 60 min (from extracted NA to result)
Number of Targets
Duplex
Internal Control
No
Storage Conditions
-85 °C to -15 °C
Regulatory Status
For research use only.
