Product Type
Kit
Unit Size
100 T
Description
The Entamoeba spp. qPCR kit has been developed specifically for the identification of Entamoeba spp. genomes in a laboratory setting. This kit has been meticulously crafted to ensure it offers the broadest possible detection range while maintaining specificity for Entamoeba spp.. Its design caters to both inclusivity and exclusivity, allowing for precise in vitro detection of this genus. Through comprehensive bioinformatic analysis utilizing NCBI reference data, the primers and probe sequences have demonstrated high (>95%) homology with a diverse array of Entamoeba spp. genomes. Notably, this kit does not detect other closely related genera.
Nucleic Acid Target
DNA
Compatibility
All standard real-time thermal cyclers
Target Species
Entamoeba species
Detection Method
Probe based
Features
Free automatic data analyzer (Fastfinder)
Components
qPCR master mix (2x)
qPCR master mix reconstitution buffer
PPMix (10x)
NTC
Positive control
DNA internal extraction control (IEC)
qPCR master mix reconstitution buffer
PPMix (10x)
NTC
Positive control
DNA internal extraction control (IEC)
Format
Lyophilized
Technology
Predesigned qPCR assays
Sample Material
DNA
Protocol Time
Approx. 60 min (from extracted NA to result)
Number of Targets
Duplex
Internal Control
No
Storage Conditions
-85 °C to -15 °C
Regulatory Status
For research use only.