Product Type
Kit
Unit Size
0.1 mL
Description
The Acanthamoeba spp quantitative qPCR standard is a highly specific product tailored for quantitative real-time PCR (qPCR) applications, specifically designed to be used alongside the Acanthamoeba spp qPCR kit. This product provides a DNA fragment sourced from Acanthamoeba species, quantified at 2 x 10^6 copies/μL. When conducting a quantitative analysis of Acanthamoeba spp. samples, it is essential to generate a standard curve using the Acanthamoeba spp quantitative qPCR standard, preparing meticulous serial dilutions with defined genome copies per microliter (copies/μL). The data obtained from these dilutions is then plotted to form a standard curve, serving as a reference for determining the concentration of Acanthamoeba spp. in the test sample. Execution of the outlined procedures is crucial in minimizing potential errors and maintaining the integrity of the testing process.
Nucleic Acid Target
DNA
Compatibility
All standard real-time thermal cyclers
Target Species
Acanthamoeba species
Detection Method
Probe based
Features
Free automatic data analyzer (Fastfinder)
Components
Quantitative standard
Positive control dilution buffer
Positive control dilution buffer
Format
Liquid
Technology
Predesigned qPCR assays
Sample Material
DNA
Protocol Time
Approx. 60 min (from extracted NA to result)
Number of Targets
Singleplex
Internal Control
No
Storage Conditions
-85 °C to -15 °C
Regulatory Status
For research use only.
