Product Type
Indirect ELISA kit
Measurement
Qualitative
Description
Anti-Trichinella spiralis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Trichinella spiralis in Human serum and plasma.
A 96-well plate has been precoated with Trichinella spiralis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Trichinella spiralis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Trichinella spiralis IgG sample captured in plate.
A 96-well plate has been precoated with Trichinella spiralis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Trichinella spiralis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Trichinella spiralis IgG sample captured in plate.
Reactivity
Human
Assay Duration
Multiple steps standard assay
Sample Type
Serum, Hep plasma, Cit plasma
Size
96 T
Detection Method
Colorimetric
Kit Components
20x washing solution: white cap, 1x50 mL.
Cover foil: 1 unit.
IgG sample diluent: colored yellow, white cap, 1x100 mL.
Stop solution: red cap, 1x15 mL.
Strip holder: 1 unit.
TMB substrate solution: yellow cap, 1x15 mL.
Trichinella spiralis (IgG) coated microplate (12x8 wells): 12 strips of 8 wells, 1 unit.
Trichinella spiralis IgG cut-off control: colored yellow, green cap, 1x3 mL.
Trichinella spiralis IgG negative control: colored yellow, blue cap, 1x2 mL.
Trichinella spiralis IgG positive control: colored yellow, red cap, 1x2 mL.
Trichinella spiralis protein A HRP conjugate: colored blue, black cap, 1x20 mL.
Cover foil: 1 unit.
IgG sample diluent: colored yellow, white cap, 1x100 mL.
Stop solution: red cap, 1x15 mL.
Strip holder: 1 unit.
TMB substrate solution: yellow cap, 1x15 mL.
Trichinella spiralis (IgG) coated microplate (12x8 wells): 12 strips of 8 wells, 1 unit.
Trichinella spiralis IgG cut-off control: colored yellow, green cap, 1x3 mL.
Trichinella spiralis IgG negative control: colored yellow, blue cap, 1x2 mL.
Trichinella spiralis IgG positive control: colored yellow, red cap, 1x2 mL.
Trichinella spiralis protein A HRP conjugate: colored blue, black cap, 1x20 mL.
Precision
Intra-assay: 3.9%. Inter-assay: 5.2%.
Platform
Microplate
Storage
Store at +4°C.
Regulatory Status
For research use only. Not for use in diagnostic procedures.
