Product Type
Indirect ELISA kit
Measurement
Qualitative
Description
Anti-Entamoeba histolytica IgG human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Entamoeba histolytica in Human serum and plasma.
A 96-well plate has been precoated with Entamoeba histolytica antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a Protein A peroxidase labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Entamoeba histolytica-specific antibodies. TMB is then catalyzed by the Protein A to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Entamoeba histolytica IgG sample captured in plate.
A 96-well plate has been precoated with Entamoeba histolytica antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a Protein A peroxidase labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Entamoeba histolytica-specific antibodies. TMB is then catalyzed by the Protein A to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Entamoeba histolytica IgG sample captured in plate.
Reactivity
Human
Assay Duration
Multiple steps standard assay
Sample Type
Cit plasma, Hep plasma, serum
Size
96 T
Detection Method
Colorimetric
Kit Components
20x washing solution: white cap, 1x50 mL.
Cover foil : 1 unit.
Entamoeba histolytica (IgG) cut-off control: colored yellow, green cap, 1x3 mL.
Entamoeba histolytica (IgG) negative control: colored yellow, blue cap, 1x2 mL.
Entamoeba histolytica (IgG) positive control: colored yellow, red cap, 1x2 mL.
Entamoeba histolytica coated microplate (12x8 wells): 12 strips of 8 wells, 1 unit.
Entamoeba histolytica protein A conjugate: colored blue, black cap, 1x20 mL.
IgG sample diluent: colored yellow. white cap, 1x100 mL.
Stop solution: red cap, 1x15 mL.
Strip holder: 1 unit.
TMB substrate solution: yellow cap, 1x15 mL.
Cover foil : 1 unit.
Entamoeba histolytica (IgG) cut-off control: colored yellow, green cap, 1x3 mL.
Entamoeba histolytica (IgG) negative control: colored yellow, blue cap, 1x2 mL.
Entamoeba histolytica (IgG) positive control: colored yellow, red cap, 1x2 mL.
Entamoeba histolytica coated microplate (12x8 wells): 12 strips of 8 wells, 1 unit.
Entamoeba histolytica protein A conjugate: colored blue, black cap, 1x20 mL.
IgG sample diluent: colored yellow. white cap, 1x100 mL.
Stop solution: red cap, 1x15 mL.
Strip holder: 1 unit.
TMB substrate solution: yellow cap, 1x15 mL.
Precision
Intra-assay: 2.1%. Inter-assay: 3.9%.
Platform
Microplate
Storage
Store at +4°C.
Regulatory Status
For research use only. Not for use in diagnostic procedures.
