The life history of Entamoeba histolytica consists of an encapsulated phase and a trophozoite phase. Its infective phase is the mature encapsulation containing the tetranuclear nucleus. Infectious encysts in fecally contaminated food or drinking water cause infection through oral ingestion. Humans are suitable hosts for Entamoeba Histolytica, and cats, dogs and rats can also be occasional hosts. Its trophozoites can invade the intestinal mucosa, phagocytose red blood cells, destroy the intestinal wall, and cause ulceration of the intestinal wall; they can also enter other tissues or organs with the bloodstream and cause extraintestinal amebiasis.

In vitro cultures of Entamoeba histolytica are important in experimental diagnosis and preservation of the worm species, and the cultures are often fecal or abscess aspirates.

Ace Therapeutics specializes in parasitology and has established a parasite culture technology platform that provides culture services for a wide range of parasites. We provide in vitro culture services for Entamoeba histolytica, aiming to facilitate its experimental diagnosis, morphological studies, life history (ecology), pathogenesis, epidemiological patterns, immunogenetic characteristics, and preservation of the species.

Service Options for Entamoeba histolytica Culture

We offer 2 methods of in vitro culture of Entamoeba histolytica, xenic culture and aseptic culture, which you can choose flexibly according to your needs.

• Xenic culture service

Robinson's medium is mainly used to culture not only the amoeba in lysate tissue, but also Entamoeba hartmani, Entamoeba coli grassi, Endolimax nana and other amoebas. We generally apply 6mL~7mL or smaller spiral covered culture tube.

• Aseptic culture service

We most commonly use BIS-33 medium (containing nutrient solution, vitamin mixture, adult bovine serum) as a liquid medium. Culture in 6ml glass covered culture tubes, as the amoeba is partly anaerobic metabolism, so the tube should be tightly covered and placed at an angle of 5°.

Service Details

Samples type: fecal, abscess aspirates or others.
Service method: xenic culture service, aseptic culture service, xenic culture & aseptic culture services
Delivery content: Entamoeba histolytica with culture medium
Test fee: please get it through online inquiry

Service Flow

1. Communicate your project requirements with us via online inquiry or by phone.
2. Sign the contract and pay the deposit.
3. Fill out the sample order and send the sample.
4. Initial test of samples. Optimize the conditions according to the customer's needs, sample characteristics, culture experience and standard methods.
5. Conduct experiments, obtain cultured parasites and make test report.
6. Delivery of the report and Entamoeba histolytica with culture medium.

Our Advantages

• 2 types of Entamoeba histolytica culture methods, with the flexibility to choose as needed.
• Professional technicians with extensive culture experience and proficiency in Entamoeba histolytica in vitro culture.
• In addition to providing Entamoeba histolytica in vitro culture services, we also provide Entamoeba histolytica testing services and animal model development services.
• In addition to our excellent service, we offer a wide range of testing products.
• Short project cycle.
• Competitive pricing.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service