Toxoplasma gondii is an intracellular parasite, also called trichuris. It is parasitic in the cells and flows with the blood to reach all parts of the body, destroying the brain, heart, and fundus of the eyes, causing the immunity of people to decline and suffer from various diseases. It is a specialized intracellular parasite that requires two hosts for its life cycle, with intermediate hosts including reptiles, fish, insects, birds, mammals and other animals and humans, and final hosts including cats and felines. Toxoplasma gondii develops in two stages, namely the extramucosal stage and the intramucosal stage. The former develops within the tissue cells of various intermediate and end-host infectious pathogens. The latter develops only in the epithelial cells of the small intestinal mucosa of the end host.

Toxoplasmosis is a worldwide human-animal parasitic disease. The construction of animal models is one of the important ways to study toxoplasmosis. The use of different animal models to explore the pathogenesis and regression of Toxoplasma gondii infection provides a good animal experimental basis for the prevention and treatment of toxoplasmosis, and has far-reaching significance for the development of human health.

Ace Therapeutics specializes in parasitology and has established a technical platform for the development of animal models of parasitic diseases, aiming to provide development and customization services for various animal models of parasitic diseases for clients worldwide. We provide animal model development services for Toxoplasma gondii. This provides a good model for the morphology, life history and especially the molecular biology of Toxoplasma gondii and the study of toxoplasmosis.

Customization Service Options for Animal Models of Toxoplasma gondii Infection

We can set up a Toxoplasma gondii disease model for you in rats or mice, it can be used for scientific research and drug research of Toxoplasma gondii. If you want to know more, you are welcome to consult us.

• Animal model of Toxoplasma gondii occult infection

Model animals: rats, 11-12 weeks old, weighing 200-220g.

Replication method: Toxoplasma gondii RH strong virulent strain tachyzoites were inoculated in the rat intraperitoneally.

Model characteristics: Infected animals were generally viable for more than 3 months. IgM antibodies gradually increased from 10 d, peaked at 70 d, and gradually decreased thereafter, while IgG gradually increased from 50 d, gradually replacing macromolecular IgM. Toxoplasma gondii activity was still present during 70 d, and gradually entered a state of recessive infection after 70 d.

Application: Asymptomatic state with worms after 70d, with long-term or even lifelong incubation in brain tissue, muscle, retina and other organs in the form of encapsulation. This model animal can be used for research on Toxoplasma gondii damage to various systems, drug screening, and diagnosis of toxoplasmosis.

• Animal model of vertical transmission of Toxoplasma gondii

Model animals: mice, BALB/c mice (8~10 weeks old).

Reproduction method: Toxoplasma gondii tachyzoites were inoculated into the peritoneal cavity of mice, and after 72 h, the peritoneal fluid of mice was aseptically drawn and was stored frozen in liquid nitrogen (after recovery for inoculation). The virgin mice were then mated with male mice in the same cage and inoculated with the recovered Toxoplasma gondii tachyzoites on the 8th day of gestation. On the 10th and 13th day of gestation, DES 1mg/100g was injected subcutaneously.

Model characteristics: The animal model of vertical transmission of Toxoplasma gondii in BALB/c mice established by this method can achieve to keep the abortion rate at a low level without all vertical transmission occurring.

Application: This model animal can be used for the study of congenital toxoplasmosis prevention and control.

Service Details

Delivery content: experiment report and Toxoplasma gondii disease animal model
Test fee: please get it through online inquiry.

Service Flow

1. Communicate your project requirements with us via online inquiry or telephone.
2. Communicate the requirements (Number, degree of infection, etc.) of Toxoplasma gondii disease animal model customization, sign the contract and pay the deposit.
3. Fill out the sample order and send the samples (optional).
4. Develop Toxoplasma gondii disease animal model and prepare project experiment report.
5. Delivery of report and animal model.

Our Advantages

• We offer mouse and rat models of Toxoplasma gondii that can be used for scientific research and drug research of Toxoplasmosis. If you have special needs, please consult us for customization.
• Our professional technicians have extensive experience in parasite animal model development.
• Advanced technology platform, scientific management system, and standard R&D procedures.
• In addition to Toxoplasma gondii animal model customization service, we also provide Toxoplasma gondii identification (Direct microscopy (blood / bone marrow / cerebrospinal fluid / thoracoabdominal fluid / sputum / bronchoalveolar lavage fluid / eye chamber fluid / amniotic fluid, etc.), biopsy (lymph node / muscle / liver / placenta, etc.), animal inoculation or tissue culture, PCR assay, DNA hybridization technique assay, staining test, intradermal assay, indirect immunofluorescence assay, ELISA assay, indirect hemagglutination assay, McAb-McAb sandwich type ELISA assay, etc.) and in vitro culture services.
• The project cycle time is short and the price is competitive.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service