Mycobacterium tuberculosis (MTB) is a major pathogen causing tuberculosis in humans and poses a serious threat. To further understand the pathogenesis of tuberculosis, there is an urgent need to study its pathogenesis, evaluate new anti-tuberculosis drugs and diagnostic reagents, and develop vaccines. The establishment of animal models of MTB infection has become an important basis for these studies. At present, a variety of animal models of TB infection have been established.

Ace Therapeutics can provide customization services for Mycobacterium tuberculosis virus models, including the mouse MTB model, guinea pig MTB model, rabbit MTB model, and chicken MTB model. These models are useful for further research on the immune response of the body after Mycobacterium tuberculosis infection, for exploring the relationship between infection and immunity in the body, and for providing new methods for effective prevention and treatment of Mycobacterium tuberculosis; they are also unique in evaluating the efficacy and safety of new tuberculosis vaccines and providing preclinical experimental data (immune data) for new tuberculosis vaccines.

Customization Service Options for Animal Models of Mycobacterium tuberculosis Infection

[Modeling mechanism] Mycobacterium tuberculosis has a variety of transmission routes to respiratory inhalation of bacterial droplets and bacterial dust as the main transmission route. Patients and sick animals are mainly aerosol transmission, laboratory commonly used mice, guinea pigs, rabbits, chickens, and other golden animals are sensitive to Mycobacterium tuberculosis of which the most sensitive animals are guinea pigs, experimental infection with Mycobacterium tuberculosis guinea pigs or mice do not produce droplets by coughing, infected animal feces, and garbage may be contaminated as a source of infectious aerosol.

[Modeling method]

Guinea pig MTB model --- Intraperitoneal injection method.

Mouse MTB model --- Tail vein injection of standard strain H37Rv. 2.5-10 million bacteria, can be about half a month of morbidity and mortality. In contrast, mongrel mice require larger doses. Different mouse strains also have differences in tolerance and susceptibility.

Rat MTB model --- Tail vein injection of standard human TB strain H37Rv.

[Model characteristics] Tail vein infection can cause a significant increase in the weight index of organs such as the lungs, liver, spleen, and kidney, and a significant decrease in the weight of the heart. In contrast, abdominal infection only showed an increase in organ weights and organ indices of the liver and spleen, and a significant increase in inguinal lymph nodes, while the rest of the organs did not differ from normal controls. The number of infected organisms did not conform to the linear relationship between body weight and organism amount in different species of animals using the same route of infection.

[Model evaluation and application] Animal models of tuberculosis play an extremely important role in studies of the immune mechanism of infection, diagnostic reagents, and drug screening, preclinical testing of vaccines, and efficacy analysis. In experimental studies of tuberculosis, guinea pigs and mice are generally considered to be the ideal experimental animals for establishing tuberculosis models. C57BL/6N is the best choice of experimental animal model for M. tuberculosis in the mouse strain. The model of non-human primates infected with TB has unique advantages in the study of TB infection and immune mechanism, vaccine immunity, etc.

Note: In addition, there are other modeling services available for you to choose from, please consult us for details.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service