Giardia lamblia, also known as pear-shaped flagellates, belongs to the class of flagellates. Giardiasis has a worldwide distribution and is also known as traveler's diarrhea because of its high incidence in travelers.

Mature tetranuclear encysts are the stage of infection, and the encysts enter the body with contaminated food and drinking water and excystation in the duodenum to form 2 trophozoites. If the trophozoite falls into the intestinal lumen and reaches the lower ileum or colonic lumen with food, it forms a cyst and is excreted with feces. Generally, only the cyst can be found in hard normal stools. Trophozoites can be found in the stool of people with diarrhea. The cyst is more resistant in the outside world and is the stage of transmission.

The pathogenic mechanism of Giardiasis is not fully understood and is related to various factors such as virulence of the strain, organismal response and symbiotic endosymbiotic environment. Mechanical blockage, nutritional competition, mechanical irritation and injury, and synergistic effects of intestinal bacteria can cause intestinal malfunction to varying degrees.

Ace Therapeutics specializes in parasitology and has established a technical platform for the development of animal models of parasitic diseases, aiming to provide development and customization services for various animal models of parasitic diseases for clients worldwide. We provide animal model development services for Giardia lamblia. This provides a good model for the morphology, life history and especially the molecular biology of Giardia lamblia and the study of Giardiasis.

Customization Service Options for Animal Models of Giardia lamblia Infection

We can construct a Giardia lamblia model for you in mice, it can be used for scientific research and drug research of Giardiasis. If you want to know more, you are welcome to consult us.

• Mice model of Giardia lamblia

Model animals: mice (SPF grade C57BL/6N mice, weight about 20 g)

Giardia lamblia source: cysts obtained by isolation and purification from fresh feces of patients and diluted with PBS.

Reproduction method: mice were gavaged with Giardia lamblia cysts. Before inoculation, mice were given metronidazole (methotrexate) to eliminate the original infected protozoa, followed by 3 d of fecal examination, and if the fecal examination was negative for cysts or trophozoites, each mouse was gavaged with encapsules.

Model characteristics: after inoculation, the incubation period of cyst discharge was 0-3d, with a cycle of 2-20d, peaking on day 6. Most of them showed interstitial bursa discharge. Pathological histology showed increased secretion on the mucosal surface of the small intestine; heavy inflammatory cell infiltration in the small intestine mucosa; edema in the interstitial tissue and submucosa; congestion of small blood vessels; and necrosis and division of the entire mucosal layer when the infection was severe. The infection rate is high (100%) compared with gerbils, which is convenient and economical.

Application: the animal model replicated by this method can be used for the study of Giardia and Giardiasis, drug screening, etc.

Service Details

Delivery content: experiment report and Giardia lamblia animal model
Test fee: please get it through online inquiry.

Service Flow

1. Communicate your requirements with us via online inquiry or telephone.
2. Communicate the requirements (Number, degree of infection, etc.) of Giardia lamblia animal model customization, sign the contract and pay the deposit.
3. Fill out the sample order and send the samples (optional).
4. Develop Giardia lamblia animal model and prepare experiment report.
5. Delivery of report and animal model.

Our Advantages

• We offer rat models of Giardia lamblia that can be used for scientific research and drug research of Giardiasis. If you have special needs, please consult us for customization.
• Our professional technicians have extensive experience in parasite animal model development.
• Advanced technology platform, scientific management system, and standard R&D procedures.
• In addition to Giardia lamblia animal model customization service, we also provide Giardia lamblia identification (Stool smear method, duodenal drainage / small intestine mucus test, biopsy tissue test, enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test (IFA), enzyme-linked immunoassay (double sandwich method, Dot-ELISA), convective immunoelectrophoresis (CIE) test, PCR test, DNA probe assay, etc.) and in vitro culture services.
• The project cycle time is short and the price is competitive.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service