Echinococcosis granulosa is a disease caused by infection with the larvae of Echinococcus granulosus (hydatid cyst / echinococtus cyst), also known as cystic echinococcosis (CE) or unilocular echinococcosis. The fine-grained echinococcosis tapeworm is a human-animal parasite whose final hosts are canine carnivores such as dogs, jackals, and wolves. The intermediate hosts are sheep, cattle, camels and other herbivores and humans. Therefore, the disease is prevalent in livestock areas, and humans can also become its intermediate hosts by accidentally ingesting eggs and developing encrustation disease. Cysts are most common in the liver, followed by the lungs, and other organs such as the brain and bones are occasionally invaded.

The incubation period of cystic echinococcosis is long, ranging from 10 to 20 years or longer from infection to disease onset. Clinical manifestations vary depending on the site of parasitization, cyst size and the presence of complications. In the early stages of infection, there are no obvious symptoms, but in the later stages, the main symptoms include coughing, wasting, stunting, rebellious coat, and prolonged recumbency, and anaphylactic shock caused by rupture of the cyst can lead to sudden death of individual animals.

As a zoonotic parasitic disease, echinococcosis has a wide distribution, wide prevalence and high incidence, and poses a greater threat to the cattle and sheep farming industry.

Ace Therapeutics specializes in parasitology and has established a technical platform for the development of animal models of parasitic diseases, aiming to provide development and customization services for various animal models of parasitic diseases for clients worldwide. We provide animal model development services for Echinococcosis granulosus. This provides a good model for the morphology, life history and especially the molecular biology of Echinococcosis granulosus and the study of Echinococcosis granulosa.

Customization Service Options for Animal Models of Echinococcosis granulosus Infection

We can establish an Echinococcosis granulosus model for you in mice, it can be used for scientific research and drug research of Echinococcosis granulosus. If you want to know more, you are welcome to consult us.

• Mice model of Echinococcosis granulosus

Model animals: KM mice of 6-8 weeks old and weighing about 20 g

Parasite source: eggs were collected from the gestational nodules of a domesticated sheep strain, and then the eggs were exfoliated to obtain six-hook larvae.

Replication methods: oral infection, intravenous infection, abdominal infection, and subcutaneous infection.

Model characteristics: Different sites of parasitization of encysts in mice can directly affect the growth rate and size of encysts. The infection rate of different strains of Echinococcosis granulosus. and different inoculums (e.g. eggs, protozoa, hexacercariae) varies for different modeled animals.

1. After 21 weeks of oral infection, the encysts were mainly parasitized in the liver and secondly in the lungs. However, the infection rate of the modeled animals was only about 80%, probably because of the short residence time of the eggs in the intestine of mice and the inability of the bile of mice to effectively digest the shell of the hexocysts, resulting in a relatively low percentage of both oral infection and the development of hexocysts into encysted larvae. However, oral infection is a more natural mode of infection and can basically reflect the natural infection status, and the cyst parasitism is close to the distribution of cysts in the intermediate hosts of natural infection.
2. After 21 weeks of intravenous infection, cysts were strictly parasitic in the thoracic cavity or on the interstitial and septal surfaces of the lung. After 180 d of infection, most of the mice died due to loss of pulmonary respiratory function caused by the large number of parasitized cysts in the lungs.
3. After 21 weeks of intraperitoneal infection, the cysts were strictly parasitic in the abdominal organs or free in the abdominal cavity.
4. In subcutaneous infections, the cysts were strictly subcutaneous and could be observed or palpated after 10 weeks of infection. However, the infection rate in model animals is about 80%, probably due to factors such as subcutaneous blood circulation and nutrient supply being less constrained than the thoracoabdominal cavity and skin muscles.

Application: This model can be used to understand the process and growth of eggs developing into encysts in the organism, and to understand the continuous immune response and antibody changes in the organism due to the invasion of eggs; it can also be used to observe the growth and development process of encysts, drug screening, observation of treatment effect and monitoring of various biological indicators such as immunology after the animals are infected with hexococci.

Service Details

Delivery content: experiment report and Echinococcosis granulosus animal model
Test fee: please get it through online inquiry.

Service Flow

1. Communicate your requirements with us via online inquiry or telephone.
2. Communicate the requirements (Number, degree of infection, etc.) of Echinococcosis granulosus animal model customization, sign the contract and pay the deposit.
3. Fill out the sample order and send the samples (optional).
4. Establish Echinococcosis granulosus animal model and prepare experiment report.
5. Delivery of report and animal model.

Our Advantages

• We offer rat models of Echinococcosis granulosus that can be used for scientific research and drug research of Echinococcosis granulosus. If you have special needs, please consult us for customization.
• Our professional technicians have extensive experience in parasite animal model development.
• Advanced technology platform, scientific management system, and standard R&D procedures.
• In addition to Echinococcosis granulosus animal model customization service, we also provide Echinococcosis granulosus identification (Intradermal test, indirect hemagglutination test, ELISA, complement binding test, latex agglutination, convective immunoelectrophoresis, immunofluorescence assay, etc. These tests can be used on a case-by-case basis.) and in vitro culture services.
• The project cycle time is short and the price is competitive.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service