Toxoplasma gondii(Toxoplasma) is an opportunistic intracellular parasite that causes disease. Toxoplasma gondii infects humans in an insidious manner, and most infected individuals with a well-developed immune system have no obvious clinical manifestations. However, in some cases of abnormal immune function, such as organ transplantation, application of immunosuppressive drugs and AIDS, serious diseases such as Toxoplasma encephalitis, Toxoplasma gondii ophthalmopathy and retinal choroid plexus infection can occur, which can even be life-threatening and cause death.

As a model organism, Toxoplasma gondii will play an important role in the study of other parasitic protozoa, and the development of gene editing technology has undoubtedly contributed to the study of Toxoplasma gondii. Efficient gene editing of Toxoplasma gondii can lay the foundation for further elucidation of the mechanism and accelerate the research progress of anti-Toxoplasma drugs and specific vaccines. The development of Toxoplasma gondii gene editing technology will certainly give a strong impetus to Toxoplasma research.

Ace Therapeutics specializes in parasitology and has established a parasite genetic engineering technology platform designed to provide genetic engineering services for a variety of parasitic diseases to clients worldwide. We can provide CRISPR/Cas9 gene editing services for Toxoplasma gondii, laying the foundation for in-depth elucidation of mechanistic studies.

CRISPR/Cas9 Genome Editing Service Options for Toxoplasma gondii

CRISPR/Cas9 technology has great application scope, it is not only limited to knockdown of target genes, CRISPR/Cas9 is also widely used in transcriptional activation and repression of genes and site-specific genetic modifications. We have established the CRISPR/Cas9 system for Toxoplasma gondii, which provides corresponding services according to different purposes. If you want to know more, you are welcome to consult us.

• Knock out service of Toxoplasma gondii gene based on CRISPR/Cas9 technology
• Knock in service of Toxoplasma gondii gene based on CRISPR/Cas9 technology
• CRISPRi service for Toxoplasma gondii gene based on CRISPR/Cas9 technology
  CRISPRi is another way to repress gene transcription other than RNAi. CRISPRi specifically silences only those genes that have no other genes in their neighborhood.
• CRISPRa service for Toxoplasma gondii genes based on CRISPR/Cas9 technology
  dCas9 can also be fused with transcriptional activators to target the promoter and enhancer regions of genes to activate endogenous expression of genes (CRISPRa). the CRISPRa approach is generally suitable for large genes that cannot be directly overexpressed exogenously.
• Epigenetic modification service of Toxoplasma gondii based on CRISPR/Cas9 technology

Service Details

Delivery content: experiment report and Toxoplasma gondii edited by CRISPR/Cas9 technology
Test fee: please get it through online inquiry.

Service Flow

1. Communicate your requirements with us via online inquiry or telephone.
2. Communicate the requirements, sign the contract and pay the deposit.
3. Fill out the sample order and send the samples (optional).
4. Establish the needed Toxoplasma gondii CRISPR/Cas9 system and prepare experiment report. (All steps from vector design, construction, activity identification, and establishment of genetically mutated Toxoplasma gondii are completed in a one-stop shop.)
5. Delivery of report and Toxoplasma gondii edited by CRISPR/Cas9 technology.

Our Advantages

• We have already had many years of successful experience in CRISPR/Cas9 system construction and activity validation.
• We have a strong technical support team and can provide free professional technical support for our customers.
• We have successfully used CRISPR/Cas9 system to provide thousands of successful cases for our customers. Our service is unanimously recognized by our customers.
• Low price and short cycle time.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service