Schistosoma mansoni is mainly parasitized in the small mesenteric veins, hemorrhoidal plexus, and occasionally in the superior mesenteric veins and intrahepatic portal vessels. The main lesions of schistosomiasis are in the colon and liver, producing egg granulomas and fibrosis, similar to but less severe than schistosomiasis japonica.

CRISPR/Cas9 is a very effective genome-targeted modification system that enables genetic manipulation such as gene masking, targeted insertion and gene repair at specific genomic loci, providing a technological innovation platform for parasite gene function analysis, drug targeting and vaccine molecular screening.

Ace Therapeutics specializes in parasitology and has established a parasite genetic engineering technology platform designed to provide genetic engineering services for a variety of parasitic diseases to clients worldwide. We can provide CRISPR/Cas9 gene editing services for Schistosoma mansoni, laying the foundation for in-depth elucidation of mechanistic studies.

Schistosoma mansoni CRISPR/Cas9 Genome Editing Service Options

We established the Schistosoma mansoni CRISPR/Cas9 genome modification method, which can effectively perform gene knockout, gene targeted knock-in and replacement, single base editing, gene activation and repression, and gene epistasis modification in Schistosoma mansoni. Based on this, the method was further developed to address the technical needs of multiple gene modifications in the study of Schistosoma mansoni gene function. If you want to know more, you are welcome to consult us.

• High-throughput sgRNA library screening service for Schistosoma mansoni
  1) Knockout library
  2) Activation library
  3) Suppression library
• Gene editing cell line customization service for Schistosoma mansoni
  1) Knockout cell lines
  2) Conditional knockout cell lines
  3) Targeted mutation cell lines
  4) Knock-in cell lines
• Off-target effect analysis service for Schistosoma mansoni
  1) Whole genome sequencing

Applications

• To study the function of Schistosoma mansoni genes.
• To study the drug resistance of Schistosoma mansoni.
• To study the drug targets of Schistosoma mansoni.
• To promote vaccine development for Schistosoma mansoni.

Service Details

Delivery content: experiment report and cell line edited by CRISPR/Cas9 technology
Test fee: please get it through online inquiry.

Service Flow

1. The client sends the experimental plan, such as knockout cells, knockout target genes.
2. Program evaluation and quotation, communication and determination of program details.
3. Communicate the requirements, sign the contract and pay the deposit.
4. Establish the needed Schistosoma mansoni CRISPR/Cas9 system and prepare experiment report. (One-stop service from gene synthesis, sgRNA design and validation, cell transfection, and sequencing validation analysis.)
5. Delivery of report and Schistosoma mansoni edited by CRISPR/Cas9 technology.

Our Advantages

• Simple and fast target design, efficient and flexible target manipulation.
• Professional technical team with more than 50% of Ph.D.s have been focusing on gene editing for many years, and can provide free professional technical support for our customers.
• One-stop service from gene synthesis, sgRNA design and validation, cell transfection, and sequencing validation analysis.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service