Babesiosis is a human-animal parasitic disease caused by parasitic blood protozoa in erythrocytes of the genus Babesia, which are infected by tick vectors. Babesia can infect humans, rats, cattle, horses, sheep, dogs, deer, birds, and other animals. The clinical symptoms of human babesiosis are mainly characterized by intermittent fever, splenomegaly, jaundice and hemolysis. Babesiosis can cause serious harm to livestock production. In order to control the damage of Babesia, a lot of research work has been carried out worldwide.

CRISPR/Cas9 is a very effective genome-targeted modification system that enables precise gene editing such as gene knockout and knock-in, providing an innovative technology for gene function analysis, drug targeting and vaccine molecular screening of parasites. The CRISPR-Cas9 system has been applied to a variety of model organisms, including human, mouse, rat, Cryptobacterium hidradenum, plant and bacteria.

Ace Therapeutics, as a genome editing service provider, is focused on leveraging CRISPR/Cas9 technology and high-throughput screening to advance basic research and the pharmaceutical industry. We have a professional technical team and a perfect genome editing platform, and have been dedicated to providing customers with a full set of genome editing solutions such as sgRNA design and validation, vector construction, gene knockout, gene knock-in, gene targeted mutation and repair, off-target effect analysis and high-throughput sgRNA library screening for many years. We can provide CRISPR/Cas9 gene editing services for Babesia, laying the foundation for in-depth elucidation of mechanistic studies.

CRISPR/Cas9 Genome Editing Service Options for Babesia

We have a technical team with rich experience in CRISPR gene editing, and we can currently realize services including gRNA design, CRISPR/Cas9 vector construction, cell transfection, positive clone identification, monoclonal cell culture, knockout cell line construction, etc. After the service is completed, we will deliver you the monoclonal cell line and detailed identification report. If you want to know more, you are welcome to consult us.

• gRNA synthesis service
• CRISPR/Cas9 vector construction
• Cas9 vector activity validation
• CRISPR knockout cell line construction
• CRISPR knock-in cell line construction

Customers need to provide

• Host cell related information (name, media formulation, transfection method)
• Target gene related information
  1) Knockout: ID of knockout gene, lethality of pre-knockout gene
  2) Knock-in: sequence of knock-in DNA, knock-in site
  3) Point mutation: point mutation location and base change

Service Details

Delivery content: experiment report, gene editing monoclonal cell lines, or Babesia animal models that edited by CRISPR/Cas9 technology
Test fee: please get it through online inquiry.

Service Flow

1. The client sends the experimental plan, such as knockout cells, knockout target genes.
2. Program evaluation and quotation, communication and determination of program details. Sign the contract and pay the deposit.
3. Establish the needed Babesia CRISPR/Cas9 system and prepare experiment report.
4. Delivery of report and products edited by CRISPR/Cas9 technology.

Our Advantages

• Systematic experimental methods, one-stop service from gRNA design, screening, vector construction to stable cell line screening.
• Authoritative detection methods and standardized service process.
• Realistic, clear and complete reports.
• We have a strong technical support team and can provide free professional technical support for our customers.
• We have successfully used CRISPR/Cas9 system to provide thousands of successful cases for our customers. Our service is unanimously recognized by our customers.

• Parasitology discovery and pre-research service
• Parasitology metabolism research service
• Parasitology target and structure function research
• Parasitology effectiveness evaluation service
• Parasitology safety evaluation service
• Parasitology pharmacological research service