Product Type
ELISA kit
Measurement
Qualitative
Description
For the qualitative determination of serum or plasma antibodies in humans, primarily IgM, to Trypanosoma cruzi using the ELISA technique.
Sample Type
Human serum or plasma
Size
96 T
Kit Components
Test strips (MT PLATE): Microwells containing Trypanosoma cruzi antigens - 96 test wells in a test strip holder.
Enzyme conjugate (CONJ): 1 bottle containing 11 mL of anti-human IgM (µ chain) Peroxidase (HRP) in a stabilizing buffer with thimerosal.
Positive control (CONTROL+): 1 vial containing 2 mL of a surrogate positive control.
Negative control (CONTROL-): 1 vial containing 2 mL of diluted negative human sera in buffer with thimerosal.
Chromogen (SUBS, TMB): 1 bottle containing 11 mL of the chromogen tetramethylbenzidine (TMB).
Wash concentrate (20x) (WASH BUF): 2 bottles containing 25 mL of concentrated buffer and surfactant with thimerosal.
Dilution buffer (SPECM DIL): 2 bottles containing 30 mL of buffered protein solution.
Stop solution (SOLN): 1 bottle containing 11 mL of 1 M phosphoric acid.
Enzyme conjugate (CONJ): 1 bottle containing 11 mL of anti-human IgM (µ chain) Peroxidase (HRP) in a stabilizing buffer with thimerosal.
Positive control (CONTROL+): 1 vial containing 2 mL of a surrogate positive control.
Negative control (CONTROL-): 1 vial containing 2 mL of diluted negative human sera in buffer with thimerosal.
Chromogen (SUBS, TMB): 1 bottle containing 11 mL of the chromogen tetramethylbenzidine (TMB).
Wash concentrate (20x) (WASH BUF): 2 bottles containing 25 mL of concentrated buffer and surfactant with thimerosal.
Dilution buffer (SPECM DIL): 2 bottles containing 30 mL of buffered protein solution.
Stop solution (SOLN): 1 bottle containing 11 mL of 1 M phosphoric acid.
Materials Provided
Trypanosoma cruzi Serology Microwell ELISA Kit
Materials Required But Not Included In Kit
MicroPipettes
Squeeze bottle for washing strips
DI water
ELISA plate reader with a 450/620-650 nm filter (optionally, results can be read visually)
Tubes for serum dilutions
Timer
Squeeze bottle for washing strips
DI water
ELISA plate reader with a 450/620-650 nm filter (optionally, results can be read visually)
Tubes for serum dilutions
Timer
Storage
Reagents, strips and bottled components should be stored at 2-8 °C.
Squeeze bottle containing diluted wash buffer may be stored at room temperature (15-25 °C).
Squeeze bottle containing diluted wash buffer may be stored at room temperature (15-25 °C).
Regulatory Status
For research use only.
