ELISA kit

Qualitative

For the qualitative screening of serum IgM antibodies to Angiostrongylus using an Enzyme Linked Immunoabsorbant Assay (ELISA) technique.

Serum

96 T

Test strips (MT PLATE): Microwells containing Angiostrongylus antigens- 96 test wells in a test strip holder.
Enzyme conjugate (CONJ): 1 bottle containing 11 mL of anti-human lgM (µ chain) conjugated to peroxidase.
Positive control (CONTROL+): 1 vial containing 2 mL of a surrogate positive.
Negative control (CONTROL-): 1 vial containing 2 mL of diluted negative human serum.
Chromogen (SUBS, TMB): 1 bottle containing 11 mL of the chromogen tetramethylbenzidine (TMB).
Wash concentrate (20x) (WASH BUF): 2 bottles containing 25 mL of concentrated buffer and surfactant.
Dilution buffer (SPECM DIL): 2 bottles containing 30 mL of buffered protein solution.
Stop solution (SOLN): 1 bottle containing 11 mL of 1 M phosphoric acid.

Angiostrongylus IgM Serology Microwell ELISA Kit

Pipettes
Squeeze bottle for washing strips (narrow tip is recommended)
Reagent grade water and graduated cylinder
Tubes for sample dilution
Absorbent paper

ELISA plate reader with a 450 nm and a 650 to 620 nm filter (optional if results are read visually).

Reagents, strips and bottled components should be stored at 2-8 °C.
Squeeze bottle containing diluted wash buffer may be stored at room temperature (15-25 °C).

For research use only.