Product Type
ELISA kit
Measurement
Qualitative
Description
For the qualitative screening of serum IgM antibodies to Ancylostoma using an Enzyme Linked Immunoabsorbant Assay (ELISA) technique.
Sample Type
Serum
Size
96 T
Kit Components
Test strips (MT PLATE): Microwells containing Ancylostoma antigens- 96 test wells in a test strip holder.
Enzyme conjugate (CONJ): 1 bottle containing 11 mL of anti-human lgM (µ chain) antibody conjugated to peroxidase.
Positive control (CONTROL+): 1 vial containing 2 mL of a surrogate positive.
Negative control (CONTROL-): 1 vial containing 2 mL of diluted negative human serum.
Chromogen (SUBS, TMB): 1 bottle containing 11 mL of the chromogen tetramethylbenzidine (TMB).
Wash concentrate (20x) (WASH BUF): 2 bottles containing 25 mL of concentrated buffer and surfactant.
Dilution buffer (SPECM DIL): 2 bottles containing 30 mL of buffered protein solution.
Stop solution (SOLN): 1 bottle containing 11 mL of 1 M phosphoric acid.
Enzyme conjugate (CONJ): 1 bottle containing 11 mL of anti-human lgM (µ chain) antibody conjugated to peroxidase.
Positive control (CONTROL+): 1 vial containing 2 mL of a surrogate positive.
Negative control (CONTROL-): 1 vial containing 2 mL of diluted negative human serum.
Chromogen (SUBS, TMB): 1 bottle containing 11 mL of the chromogen tetramethylbenzidine (TMB).
Wash concentrate (20x) (WASH BUF): 2 bottles containing 25 mL of concentrated buffer and surfactant.
Dilution buffer (SPECM DIL): 2 bottles containing 30 mL of buffered protein solution.
Stop solution (SOLN): 1 bottle containing 11 mL of 1 M phosphoric acid.
Materials Provided
Ancylostoma Serology Microwell ELISA Kit
Materials Required But Not Included In Kit
Pipettes
Squeeze bottle for washing strips (narrow tip is recommended)
Reagent grade water and graduated cylinder
Tubes for sample dilution
Absorbent paper
Squeeze bottle for washing strips (narrow tip is recommended)
Reagent grade water and graduated cylinder
Tubes for sample dilution
Absorbent paper
Suggested Materials
ELISA plate reader with a 450 nm and a 650 to 620 nm filter (optional if results are read visually).
Storage
Reagents, strips and bottled components should be stored at 2-8 °C.
Squeeze bottle containing diluted wash buffer may be stored at room temperature (15-25 °C).
Squeeze bottle containing diluted wash buffer may be stored at room temperature (15-25 °C).
Regulatory Status
For research use only.
